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Chemical Separation of Cellular Components from Tissue:Isolation

机译:细胞组分与组织的化学分离:分离

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Solubilization of rat liver cells with sodium dodecyl sulfate (SDS) solutions was studied. Complete membrane solubilization occurs in 1 and 2 wt % SDS concentrations within 18 hr at 37℃. The relative viscosities of DNA recovered from fresh, lyophilized and homogenized rat liver tissue reveal that the latter fragment DNA strands. The extraction-centrifugation recovery process leaves a commercially prepared DNA intact. Requirements for foam fractionation of the solubilized cellular components were also examined.

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