The Mechanism of Photorepair of UV Damage by Photolyase May 1, 1973 – April 1, 1976

摘要

During the past three years our primary objective has been to prepare photolyase from Baker's yeast in a homogeneous state in order to begin characterization of the enzyme chemically and physically. Our secondary goal has been to isolate and chemically characterize a pure substance from Baker's yeast that increases the activity of a highly purified photolyase preparation (8,9). This substance, which we refer to as an activator, might be the one absorbing the effective photoreactivating wavelengths or might enhance the reaction by some unknown mechanism. Certainly, purifying it and learning how it increases photolyase activity would contribute to our basic understanding of photoreactivation. With pure enzyme we could also address the problem of how the enzyme recognizes its substrate and complexes it.