Enzymatic Induced Unmasking of Fetal Antigens on Normal Murine Hemopoietic Stem Cell Surfaces

摘要

Based upon information provided by indirect immunofluorescence and x-ray fluorescence, this investigation has demonstrated that fetal determinants are expressed upon the cell surface of adult cells, specifically; normal adult murine hemopoietic stem cells, derived from the bone marrow of BALB/c and C57 mice. This study has demonstrated that via enzymatic modification of the cell surface by collagenase followed by neuraminidase, these normal adult cells, which displayed no fetal determinants prior to enzyme treatment, have their membrane surface unmasked by some mechanism(s) and now express common fetal liver determinants. The specificity of these determinants is assayed through use of an antiserum which has been prepared against murine fetal liver (rabbit anti mouse fetal liver serum). These cells do not display these determinants when treated with neuraminidase followed by collagenase. (ERA citation 03:057593)