Attempts to Apply Affinity Labeling Techniques to Ribulosebisphosphate Carboxylase/Oxygenase

摘要

Studies on carboxylases/oxygenases from different species may be necessary to confirm that a residue implicated as essential is indeed an active-site component. To provide an especially stringent test case for the identification of species invariant structural features the enzymes from two phylogenetically distant species, spinach and Rhodospirillum rubrum, were compared. To date, the reactions of Br-butanone-P sub 2 and BrAcNHEtOP with the spinach enayme have been rather thoroughly characterized; only preliminary experiments have been completed with the R. rubrum enzyme. Both enzymes were isolated and assayed for carboxylase activity (spectrophotometrically or exp 14 CO sub 2 -fixation) and for oxygenase activity. (ERA citation 03:053549)