View on Techniques of Preparation of Cell and Tissue Culture Specimens for Electron Microscopy.

摘要

Many investigators fix and embed cell cultures in situ for electron microscopy. Maximum structural integrity is maintained,and cell-to-cell contacts are easily studied. Coating coverslips with evaporated carbon is a popular method for allowing easy separation of polymerized blocks from the coverslips. Because carbon coatings are hydrophobic,we have developed a new method whereby the carbon coating is overlaid with evaporated silicon monoxide,providing a surface that wets easily and still allows separation of polymerized blocks from the coverslip. Our complete technique for coating coverslips,fixation,dehydration,and embedding is described. (ERA citation 03:022233)