CHO/HGPRT Mutagenicity Assay. I. Experimental Procedure

摘要

Efforts to develop such a system utilizing Chinese hamster ovary (CHO) cells and the purine analogue 6-thioguanine (TG) to quantify mutation induction at the hypoxanthine (Hx)-guanine phosphoribosyl transferase (HGPRT) locus are described. These studies include the definition of such parameters as medium, mutagen treatment conditions, subculture protocol, phenotypic expression time, and optimal conditions for selection of the mutant phenotype. The selection conditions have been established in terms of medium, serum, concentration of selective agent, and cell density. The system has been used to determine the mutagenicity of various physical and chemical agents. (ERA citation 04:052198)