DNA Excision Repair in Escherichia Coli.

摘要

Studies on excision repair of ultraviolet irradiated (uv) DNA in the bacterium Escherichia coli are summarized. A method was established for the isolation and partial purification of the proteins required for the initial steps in the repair process. These proteins, which are the products of the uvrA exp + , uvrB exp + , and uvrC exp + genes, were shown to interact to cause strand cleavage of DNA damaged by uv and chemicals. The endonuclease reconstituted from the partially purified components are basically different from other repair endonucleases previously characterized in its complexity, cofactor requirement, and broad substrate specificity towards bulky type of lesions in DNA. (ERA citation 07:051743)