Separation of Chinese Hamster Chromosomes by Velocity Sedimentation at Unit Gravity

摘要

New techniques are described for the separation of isolated Chinese hamster M3-1 chromosomes by velocity sedimentation at unit gravity. Up to 10 exp 10 chromosomes were sedimented on simple continuous hexaleneglycol-sucrose gradients. During the sedimentation process non-disrupted cells, nuclei, cellular debris, and damaged chromosomes were easily separated from the intact chromosomes. In addition, the chromosomes themselves were separated into several fractions by size. The chromosomes types in each fraction were determined by flow cytometry. (ERA citation 05:026373)