Orientation and Energy-Transfer Studies on Chlorophyll in the Photosynthetic Membrane

摘要

The two methods of study used for the light reactions of photosynthesis are orientation dependent spectroscopy and picosecond resolution of the fluorescence decay kinetics. Analysis of spectroscopic measurements on complex partially ordered ensembls, such as photosynthetic systems, is usually limited by knowledge of the orientational distribution function. A new method of parametrically representing the distribution function using a physical model of the partially ordered ensemble is described. The parametric representation of the distribution function is the density of states function. Many formulas are included which can be used to calculate density of state functions for a large range of problems. Fluorescence decay kinetics in chloroplasts from green plants and algae are investigated using a synchronously pumped, mode-locked dye laser as an excitation source.