Isolation of a Somatomedin Binding Protein from Human Preterm Amniotic Fluid: Development of a Radioimmunoassay

摘要

This thesis investigates the nature and biological behaviour of a somatomedin binding protein, identified in preterm amniotic fluid (AF). For that purpose a double antibody radioimmunoassay was developed. Purified AF binding protein (AFBP) was iodinated by the chloramine-T method, and dilutions of partially purified AFBP were designated as the standard, with the results expressed in mu g equivalent protein/ml. The sensitivity of the assay was improved by adoption of the nonequilibrium procedure. AFBP values were twice as high in preterm AF as in term AF. Includes Dutch summary; 182 refs.; 42 figs.; 10 tabs. (Atomindex citation 17:047229)