Genetic and Developmental Study of a Complex Locus in the House Mouse. Final Report, 1976-1983

摘要

More than 50 T/t-complex and other mouse chromosome 17 variants have been maintained and used for genetic study. Congenic strains of t-mutants on C3H, 129/TER and LT/sv backgrounds have been constructed. We have continued experiments to extract and analyze t-haplotypes from wild populations of mice, and to define and man other chromosome 17 genes. Most recently we have carried out experiments to map the lethal factors within t-haplotypes relative to one another, utilizing recombination that we have found to occur freely between two different complementing t-haplotypes. These experiments not only defined different t-lethal mutations as non-allelic but showed that several are in very close physical association with the H-2 locus, which is itself transposed or inverted to an anomalous position in t-haplotypes. This information led to restriction enzyme mapping that showed the molecular structure of all t-haplotypes to be much more similar to one another than wild type chromosomes are to one another. Future experiments will include chromosomal walking with cloned DNA from t-haplotypes to further define their structure. (ERA citation 08:039404)