Response of CHO Cell DNA Polymerase alpha to DCTP and DTTP Pool Imbalance: Relation to DNA Synthesis Inhibition, Survival, and Mutation.

摘要

Utilizing a deoxynucleoside triphosphate (dNTP) pool-altered, polymerase alpha -altered mutant of CHO-K1 and a permeable cell-DNA synthesis system, the study examines the complicated interrelationship of dNTP pools as potential regulators as well as substrates for polymerase alpha . These findings are then related to the commonly observed uv-induced inhibition of DNA synthesis, and, ultimately, to the biological consequences of exposure to radiation and chemical insults. (ERA citation 08:039396)