Capillary Zone Electrophoresis: Instrumentation and Use with Nonaqueous Solvents

摘要

Capillary zone electrophoresis was performed in open tubular fused silica capillaries as small as 60 mu m inside diameter at applied voltages up to 30 kV. On-column detectors based on ultraviolet absorbance and molecular fluorescence were designed and constructed. Migration of polynuclear aromatic hydrocarbons (PNA) was observed in aqueous as well as nonaqueous media. Electroosmotic flow allowed separation of compounds by differential migration. Retention times decreased as medium polarity increased. Retention times ranged from 2 to 15 minutes for PNAs. Efficiency was considerably greater in aqueous media although resolution was greater in nonaqueous media. (ERA citation 09:023026)