Modification of Thermal Sensitivity of Chinese Hamster Cells by Exposure to Solutions of Monovalent and Divalent Cationic Salts

摘要

Chinese hamster V79 cells were heated in culture medium or in 0.155-mol.dm exp -3 solutions of LiCl, NaCl, KCl, MgCl sub 2 , CaCl sub 2 and BaCl sub 2 . The presence of any one of these ionic solutions during heating increased the thermal sensitivity of the cells. The order of increased thermal sensitivity was KCl > LiCl > NaCl for the monovalent salts and BaCl sub 2 > MgCl sub 2 > CaCl sub 2 for the divalent cation salts. The addition of glucose to LiCl or NaCl solutions did not reduce the thermal sensitization caused by these solutions. When cells were sensitized by LiCl or NaCl treatment, a change in pH from 7.2 to 6.6 did not further increase thermal sensitivity. These data show that nutrient and ionic factors and their interplay are involved in cellular thermal sensitivity. (Atomindex citation 16:041476)