Mechanism of the Iron-Containing Superoxide Dismutase from E. Coli

摘要

Considerable progress has recently been made in elucidating the structure-function relations of the iron-containing superoxide dismutase (FeSD) from Escherichia coli: A three-dimensional structure to 3 A resolution was reported, the sod B gene has been cloned and over-expression of the protein observed, and a comprehensive mechanism has been proposed. In this communication a brief review is presented of the previous mechanistic work, describe the effect of D sub 2 O on the kinetic parameters, and present a preliminary analysis of the effect of added salts and ionic strength on the activity of E. coli FeSD. 10 refs., 1 fig., 1 tab. (ERA citation 11:009828)