Role of Ca exp 2+ /Calmodulin in Phosphorylation of Proteins in Plants. Final Report, 1 August 1983-31 July 1985

摘要

In order to elucidate the role of Ca exp 2+ -dependent protein phosphorylation in plant cell function, a Ca exp 2+ -dependent protein kinase from suspension-cultured soybean cells has been partially purified and characterized. It was previously shown that crude extracts of the soybean cells contain soluble protein kinase(s) that preferentially phosphorylates at least eight endogenous proteins in vitro in the presence of 0.5 mM CaCl sub 2 . An assay based on phosphorylation of the artificial substrate histone H1 has been devised. 26 refs., 4 figs. (ERA citation 11:001367)