Determination of the Steady-State Behavior of Immobilized beta -Galactosidase Utilizing an Integral Reactor Scheme

摘要

Analysis of the steady-state behavior of immobilized beta -galactosidase by integral reactor techniques has yielded a model which allows one to predict reactor performance under normal operating conditions. Values of the mechanistic rate constants for enzymatic hydrolysis of lactose were determined as a function of temperature by fitting the reactor model to experimental lactose conversion profiles. Use of this model along with the activity decay characteristics of the immobilized enzyme which have been presented in a previous publication could prove to be a useful tool in determining appropriate operating strategies for industrial applications of the immobilized enzyme catalyst. 18 refs., 5 figs., 6 tabs. (ERA citation 10:033460)