Three-Dimensional Reconstruction and Data Display of Chromosome Structure Studied by Electron Microscope Tomography

摘要

Modern transmission electron microscopes can be employed to generate useful and informative three-dimensional reconstructions of asymmetric cell structures by adapting the principles of axial tomography and utilizing the power of digital image processing. An example of this technique, called electron microscope tomography (EMT), applied to analysis of the 3-D structure of an active eukaryotic gene in situ is presented. Future improvements in the field of EMT depend upon the development of methods for visualizing, editing and interpreting the rich image information of the 3-D reconstructions. 2 refs., 5 figs. (ERA citation 10:032228)