Reconstitution of LHC Phosphorylation by a Protein Kinase Isolated from Spinach Thylakoids

摘要

Protein kinase activity is responsible for phosphorylating the (LHC) light-harvesting chlorophyll a/b protein complex of photosystem II, leading to its migration in the thylakoid membrane, the fractional redistribution of excitation energy between photosystems II and I, and the phenomenon of state transition. Previous work from this laboratory described the purification to homogeneity of a thylakoid protein kinase which catalyzes the phosphorylation of isolated LHC at 1-10% of a rate estimated for this enzyme and substrate when resident together in the thylakoid membrane. In this communication, we report rates of LHC phosphorylation that are close to physiological, in a system comprised of isolated purified protein kinase (LHCK) and native LHC. 9 refs., 1 fig., 2 tabs. (ERA citation 12:000293)