Preliminary Studies on Molecular Characterization of Two New Pseudomonas Species Capable of Utilizing Chloroaromatic Compounds

摘要

Two new strains of Pseudomonas were isolated which can utilize chlorophenoxy alkanoic acids as their sole source of carbon and energy. These Pseudomonas isolates were obtained through the process of selective enrichnment from a mixed microbial population of a municipal activated sludge treatment plant. The kinetics of detoxification of 2,4-D and 2,4,5-T by these two new isolates appear to be very promising and makes them potential candidates for use in bioprocessing of the toxic agent. Preliminary studies on the molecular biology of these species indicate that they contain plasmids and their dehalogenase enzyme mixture in crude cell free extract can detoxify chloroaromatic compounds. 10 refs., 1 fig., 3 tabs. (ERA citation 11:052240)