Investigation of Molecular Mechanisms in Photodynamic Action and Radiobiology with Nanosecond Flash Photolysis and Pulse Radiolysis: Final Report for Period April 1, 1972-June 30, 1987

摘要

Laser flash was employed to investigate initial photolysis mechanisms in enzymes and constituent aromatic amino acids. The key role of photoionization and solvated electron generation was demonstrated. The photochemistry of furocoumarins employed for phototherapy of skin diseases was investigated, emphasizing the production and subsequent reactions of singlet molecular oxygen. The photochemistry of porphyrins employed for photodynamic therapy of malignant tumors was studied, emphasizing photosensitization of model membranes, liposomes and resealed red blood cell membranes. Photosensitization in light-scattering media was investigated in a tissue model, consisting of polystyrene microspheres in an aqueous dye solution and analyzed with the diffusion approximation to radiative transfer. The diffusion approximation was employed to develop a dosimetry model for photodynamic therapy. (ERA citation 12:042192)