High Resolution-Sensitivity Characterization of Polycyclic Aromatic Hydrocarbon-DNA Adducts Using Fluorescence Line Narrowing Spectrometry

摘要

The application of fluorescence line narrowing spectrometry (FLNS) to the investigation of polar polycyclic aromatic hydrocarbon (PAH) metabolites and their corresponding DNA adducts is demonstrated. The selectivity is shown through the successful resolution of all components in separate mixtures of similar but distinct derivatives of benzo(a)pyrene, benz(a)anthracene, and chrysene. The separate mixtures were composed of six metabolites, five DNA adducts, each metabolite and its corresponding DNA adduct, and six metabolites and two DNA adducts. The broad applicability of FLNS is demonstrated through applications to the analysis of globin adducts, PAH metabolites in urine, and real samples, and to the investigation of carcinogenic metabolic pathways. 98 refs., 31 figs., 5 tabs. (ERA citation 14:017907)