Supercritical Fluid Chromatography: The Effect of the Partial Molar Volume in the Stationary Phase on Retention

摘要

Retention in supercritical fluid chromatography (SFC) is a dynamic mechanism involving intermolecular interactions in the mobile phase and stationary phase. A simple thermodynamic model describing solute retention has been investigated based on the macroscopic thermodynamic variables of the solute partial molar volume in the stationary and mobile phases. Solute-solvent interactions in the mobile phase are dominant at lower pressures. Solute interaction with the five different stationary phases reflects the polarizability difference between these phases as shown by the partial molar volume of the solute in the stationary phase. 12 refs., 4 figs. (ERA citation 13:054384)