Genomic Sequence Comparisons: Progress Report, August 1, 1987-April 7, 1988

摘要

A multiplex sequencing method for DNA has been refined and tested. DNA fragments strategically tagged by ligation to a small set of unique oligonucleotides are pooled and subjected to genomic sequencing. Chemical sequencing reactions have been expedited by adaptation to use of 96 well plates. The electrophoretically separated products are transferred from gels to nylon membranes. Each membrane can yield a quantity of data equivalent to that from one conventional sequencing gel multiplied by the number of probes used. The maximum number of probings per membrane has increased from 9 to 52. Throughout these probings both signal strength and image quality are retained at their original levels which suggests that the upper limit may be still higher. The number of membranes which can be simultaneously probed per day has increased from 8 to 81. (ERA citation 13:055069)