Use of an Antikinetochore Antibody to Discriminate Between Micronuclei Induced by Aneuploidogens and Clastogens.

摘要

Investigators have reported using antikinetochore antibodies to demonstrate the presence of kinetochores in micronuclei. This approach has also been combined with the cytokinesis-block method to develop a rapid in vitro assay for aneuploidy, which has been shown to work very well in both human lymphocytes and Chinese hamster ovary (CHO) cells. The assay is based upon the assumption that a kinetochore-positive micronucleus contains the centromere and presumably the entire chromosome, while kinetochore-negative micronuclei, regardless of their size, contain only chromosome fragments. In this paper we examine in more detail the micronuclei contained in the cells we reported on previously. The fraction of kinetochore-positive and -negative micronuclei are shown to reflect the mechanism of action for various agents tested in human peripheral lymphocytes and CHO cells.