Development of Critical Life Stage Assays: Teratogenic Effects of Ash Basin Effluent Components on Freshwater Fish, Gambusia Affinis and Daphnia: Progress Report, 21 May 1988-1 June 1989.

摘要

This project will establish a method for describing and evaluating the reproductive level of the Gambusia in the natural state, i.e., non-laboratory establishment of pregnancy. An additional aspect of the overall problem of establishing the natural level of the fertility of free ranging fish has been initiated with the inclusion of another Savannah River Plant (SRP) pond, (Par Pond), and three non-SRP ponds (Edgar Brown Pond, Lebby's Pond, Duncan's Pond) to the previously studied ponds (Ash Basin and Risner Pond). It is necessary to have a wider range of environments than that provided by a single comparison, e.g., Ash Basin versus Risher Pond, to be able to evaluate any one ponds' effect on the life phase of the Gambusia. To begin one of the major objectives of the research project proposal, the investigators have ordered embryonated eggs of the Medaka fish from a commercial source. Even though the Gambusia is a live bearer and the Medaka is an egg bearer, these latter fish eggs will allow the investigators to begin to learn the approximate magnitude of concentrations of inorganic (and possibly organic organic) substances to add to the developing embryonic system in order to study their effects on either the egg-enclosed embryo or the female fish-enclosed embryo. Obviously, the two systems are significantly different but it is anticipated that interesting information will be obtained from a comparison of results in the two systems. 9 figs., 1 tab.