Construction and evaluation of a regenerable flouroimmunochemical-based fiber optic biosensor.

摘要

A microscale fiber optic biosensor that is capable of in situ regeneration is described and characterized. By combining recently developed fiber optic sensing technology with a capillary column reagent delivery system, it is possible to perform a variety of benchtop affinity assay procedures repetitively and remotely. The configuration of the sensing chamber at the terminus of the fiber is an important design feature. The construction and operation of the sensor is described and the results of evaluations of the sensor using an antibody/antigen system are presented. Affinity assay steps such as the delivery of solid phase affinity reagents, secondary reagents, and rinse solutions are demonstrated. Sampling is accomplished by mild aspiration. Coefficients of variation (CVs) for these steps are all less than 10. The capability of selectivity measuring fluorescently-labeled anti-rabbit-immunoglobulin G (IgG), in the present of a similar protein, utilizing its immunospecific interaction with rabbit-IgG beads, is demonstrated and exhibits a CV of 6.2. A near linear calibration plot is presented over a concentration range of 0.011 mg ml(sup (minus)1) (approximately the limit of detection) to 0.11mg ml(sup (minus)1). 30 refs., 4 figs., 2 tabs.