Metabolites of IPPA, BMIPP, and DMIPP fatty acids in rats hearts: A quantitative HPLC-study.

摘要

The significance of the use of radioiodinated fatty acid analogues such as 15-(p-iodophenyl) pentadecanoic acid (IPPA), 15-(p-iodophenyl)-3-R,S-methylpentadecanoic acid (BMIPP), and 15-(p-iodophenyl)-3-dimethylpentadecanoic acid (DMIPP) for the evaluation of regional myocardial fatty acid uptake is well documented. An understanding of the relative incorporation of these fatty acid analogues into various fatty acid pools is important to correlate fatty acid uptake and release with flux through the various metabolic pathways. While the free fatty acid (FFA) pool is immediately available for oxidation to meet energy demands for contraction under normoxic conditions, the FFA are also stored as triglycerides (TG) for oxidation at a later period. Although the incorporation of IPPA, BMIPP, and DMIPP into the major phospholipid classes has not been previously investigated, demonstration of the incorporation of IPPA and BMIPP into phospholipids would be important as further evidence that these ''modified'' fatty acids are, at least qualitatively, metabolized through the usual metabolic pathways. The goals of the present studies were thus to develop the necessary HPLC techniques and evaluate the incorporation of IPPA, BMIPP, and DMIPP into complex endogenous lipids in dual label studies. 2 refs., 6 figs.