Molecular biological enhancement of coal desulfurization: Cloning and expression of the sulfoxide/sulfone/sulfonate/sulfate genes in (ital Pseudomonads) and (ital Thiobacillae). Fifth quarterly report, July-September 1990.

摘要

Research continued on coal desulfurization and the study of biological enhancement of desulfurization. This quarters work included: crosses between soil isolates containing r68.45 and P. aeruginosa 27853-2a; extents of crosses and abundances of transconjugants; purpose of repeated crosses; noteworthy features of mating protocol; phenotypic peculiarities and phenotypic stabilities of transconjugants; characterization of antibiotic sensitivity; effect of receiving R68.45 from P. putida PRS 2003; transfer of R68.45 from C18 to auxotrophs derived from P. aeruginosa 27853; and differences in ability of various soil isolates to transfer Dbt(sup +) phenotype to recipient cells; incipient characterizations of Thiobacillae spp. growth and antibiotic sensitivity; plasmid isolation; and acquisition of new soil isolates.