首页> 美国政府科技报告 >Characterization and modification of phage T7 DNA polymerase for use in DNA sequencing. Summary progress report, July 1, 1990-June 30, 1991.

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Characterization and modification of phage T7 DNA polymerase for use in DNA sequencing. Summary progress report, July 1, 1990-June 30, 1991.

机译：表征和修饰用于DNa测序的噬菌体T7 DNa聚合酶。摘要进度报告，1990年7月1日至1991年6月30日。

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This project focuses on the phage T7 DNA polymerase (gene 5 protein) and its accessory proteins for use in DNA sequence analysis. Crystallization of the gene 5 protein/thioredoxin complex is underway. We have genetically modified the gene 5 protein to eli ...

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作者
C. C. Richardson;
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年度 1991
页码 1-9
总页数 9
原文格式 PDF
正文语种 eng
中图分类工业技术;
关键词
AIDS Virus; Amino Acid Sequence; Bacteriophages; DNA Helicases; DNA Polymerases; DNA Replication; DNA Sequencing; DNA-ase; EDB/550200; EDB/550400; Electrophoresis; Escherichia Coli; Magnesium Ions; Manganese Ions; Molecular Biology; Mutagenesis; Progress Report; Protein Engineering; Protein Structure; Pyrophosphates; RNA Processing; RNA-ase; Structure-Activity Relationships;

机译：艾滋病病毒;氨基酸序列;噬菌体; DNa解旋酶; DNa聚合酶; DNa复制; DNa测序; DNa酶; EDB / 550200; EDB / 550400;电泳;大肠杆菌;镁离子;锰离子;分子生物学;诱变;进展报告;蛋白质工程;蛋白质结构;焦磷酸盐; RNa加工; RNa-ase;结构 - 活性关系;

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专利

1. Validation of phage T7 biological dosimeter by quantitative polymerase chain reaction using short and long segments of phage T7 DNA [J] . Hegedus M., Modos K., Ronto G., Photochemistry and Photobiology: An International Journal . 2003,第3期

机译：通过使用噬菌体T7 DNA的短片段和长片段的定量聚合酶链反应验证噬菌体T7生物剂量计
2. Arabidopsis thaliana organelles mimic the T7 phage DNA replisome with specific interactions between Twinkle protein and DNA polymerases Pol1A and Pol1B [J] . Stewart A. Morley, Antolín Peralta-Castro, Luis G. Brieba, BMC Plant Biology . 2019,第1期

机译：拟南芥拟南芥细胞器模仿T7噬菌体DNA的特异性相互作用，闪光蛋白和DNA聚合酶POL1A和POL1B之间的特异性相互作用
3. Full shut-off of Escherichia coli RNA-polymerase by T7 phage requires a small phage-encoded DNA-binding protein [J] . Nucleic Acids Research . 2017,第13期

机译：通过T7噬菌体完全关闭大肠杆菌RNA聚合酶需要小噬菌体编码的DNA结合蛋白
4. Study on the Fidelity of Biodevice T7 DNA Polymerase [C] . Ming Li, Zhong-Can Ou-Yang, Yao-Gen Shu International Joint Conference on Biomedical Engineering Systems and Technologies . 2018

机译：生物维思T7 DNA聚合酶保真性研究
5. Characterization of DNA structural elements and conformational changes accompanying the binding of T7 RNA polymerase to its promoter. [D] . Ujvari, Andrea. 1999

机译：T7 RNA聚合酶与其启动子结合后，DNA结构元件的特征和构象变化。
6. T7 DNA polymerase in automated dideoxy sequencing. [O] . T Kristensen, H Voss, C Schwager, 1988

机译：自动双脱氧测序中的T7 DNA聚合酶。
7. Characterization and modification of phage T7 DNA polymerase for use in DNA sequencing. Final report, June 1, 1988--January 31, 1996 [O] . Richardson, C.C. 1996

机译：表征和修饰用于DNa测序的噬菌体T7 DNa聚合酶。最终报告，1988年6月1日至1996年1月31日
8. Characterization and modification of phage T7 DNA polymerase for use in DNA sequencing. Progress report, September 16, 1989-June 30, 1990. [R] . Richardson, C. C. 1990

机译：表征和修饰用于DNa测序的噬菌体T7 DNa聚合酶。进展报告，1989年9月16日至1990年6月30日。

Characterization and modification of phage T7 DNA polymerase for use in DNA sequencing. Summary progress report, July 1, 1990-June 30, 1991.

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