Separation of Highly Complex Mixtures by Two-Dimension Liquid Chromatography, (Final Report)

摘要

This report summarizes the progress made on the title project during the grant period. We developed a new classification of two-dimensional separations based on the observation that separations can be made in time or in space. Thus, two-dimensional separations can be made in timeOEtime, spaceOEspace, spaceOEtime, or timeOEspace. The two successive separations must use two different modes of chromatography that afford uncorrelated or weakly correlated patterns of retention factors for the components of the samples analyzed. Our attention was mainly focused on the separation of protein digests, particularly, on those of the digests of myoglobin and bovine serum albumin as model systems and extremely efficient temporal separations were developed. We also designed and constructed new instruments to carry out spaceOEspace separations (True Bidimensional Chromatography, HPLC2 or spacial separations) and timeOEspace separations (a new hybrid combination of a temporal and a spacial separation that we designed).