Capillary electrophoresis - electrospray ionization mass spectrometry in small diameter capillaries.

摘要

Methods (such as small inner diameter capillaries) are being explored to increase analyte sensitivity in capillary electrophoresis- electrospray ionization/mass spectroscopy(CE-ESI/MS). Results are reported for melittin in a protein mixture, with 10 to 100 (mu)m ID capillaries; and for a mixture of aprotinin, cytochrome c, myoglobin, and carbonic anhydrase, with 5 to 50 (mu)m ID capillaries. It is shown that an increase in solute sensitivity occurs when small ID capillaries ((lt) 20 (mu)m) are used in CE-ESI/MS for both a peptide and a protein mixture. 3 figs. (DLC)