WetLab-2: Wet Lab RNA SmartCycler Providing PCR Capability on ISS.

机译：WetLab-2：Wet Lab RNa smartCycler在Iss上提供pCR功能。

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摘要

The WetLab-2 system will provide sample preparation and qRT-PCR analysis on-board the ISS, a capability to enable using the ISS as a real laboratory. The system will be validated on SpX-7, and is planned for its first PI use on SpX-9.

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作者
Parra, M.; Schonfeld, J.;
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年度 2015
页码 1-11
总页数 11
原文格式 PDF
正文语种 eng
中图分类工业技术 ;
关键词
International space station; Space laboratories; Spaceborne experiments; Polymerase chain reaction; Real time operation; Gene expression; Ribonucleic acids; Exobiology; Commercial off-the-shelf products; Experiment design; Fluidics; Cell culturing;

机译：国际空间站;空间实验室;星载实验;聚合酶链反应;实时操作;基因表达;核糖核酸;外生物学;商业现货产品;实验设计;流体学;细胞培养;

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3. Quantitative Real-Time PCR as a Novel Detection Method for Micro-RNAs Expressed by Cervical Cancer Tissue: A Review [J] . Rashmi Nathasha Wickramasinghe, Nakandala Darshana Suraj Goonawardhana, Samanthi Priyanganee Premaratne, 生物科学与医学（英文） . 2021 ,第009期
4. Identification of stable internal control genes for accurate normalization of real-time quantitative PCR data in testicular tissue from two breeds of cattle [J] . Pradeep Nag, Ankur Sharma, Elango Kamaraj, 亚太生殖杂志（英文版） . 2020 ,第005期
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6. Comparison of SmartCycler Real-Time Reverse Transcription-PCR Assay in a Public Health Laboratory with Direct Immunofluorescence and Cell Culture Assays in a Medical Center for Detection of Influenza A Virus [J] . Nadia F. Habib-Bein, William H. Beckwith, Donald Mayo, Journal of Clinical Microbiology . 2003 ,第8期

机译：在公共卫生实验室中对SmartCycler实时逆转录PCR-PCR检测与直接免疫荧光检测和在医学中心检测甲型流感病毒的细胞培养检测进行比较
7. Wet-lab Tested MicroRNA Assays for qPCR Studies with SYBR? Green and DNA Primers in Pig Tissues [J] . Caroline M.J. Mentzel, Kerstin Skovgaard, Sarai Córdoba, MicroRNA . 2014 ,第3期

机译：经SYBR？进行qPCR研究的经湿实验室检验的MicroRNA分析猪组织中的绿色和DNA引物
8. Multiplex Real-Time PCR Melting Curve Assay for Identifying HPV Types Using the Cepheid SmartCycler [J] . Deharvengt S. J., Turner S. A., Kennedy L. S., The Journal of molecular diagnostics: JMD . 2016 ,第6期

机译：使用造父变星SmartCycler鉴定HPV类型的多重实时PCR熔解曲线测定
9. Quantitative Nucleic Acid Analysis Using Digital PCR: Absolute Counting of DNA (Examples Including Liquid Biopsies in Cancer) and RNA (mRNA and miRNA Using One-Step RT-dPCR) [C] . Michael Samuels, Leonora Balaj, Ben Ho Park, Molecular Medicine TRI-CON. . 2014

机译：使用数字PCR的定量核酸分析：DNA的绝对计数（癌症中的液体活检）和RNA（使用单步RT-DPCR的mRNA和miRNA）
10. Biospecimen RNA Quality Control in Reverse-Transcription, Quantitative PCR (RT-qPCR) Clinical Tests. [D] . Stanoszek, Lauren M. 2015

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11. Comparison of SmartCycler Real-Time Reverse Transcription-PCR Assay in a Public Health Laboratory with Direct Immunofluorescence and Cell Culture Assays in a Medical Center for Detection of Influenza A Virus [O] . Nadia F. Habib-Bein, William H. Beckwith III, Donald Mayo, 2003

机译：在公共卫生实验室中对SmartCycler实时逆转录PCR-PCR检测与直接免疫荧光检测和在医学中心检测甲型流感病毒的细胞培养检测进行比较
12. Wet-lab tested microRNA assays for qPCR studies with SYBR®Green and DNA primers in pig tissues [O] . Mentzel Caroline M. Junker, Skovgaard Kerstin, Córdoba Sarai, 2014

机译：湿实验室测试microRNa检测用于猪组织中sYBR®Green和DNa引物的qpCR研究

WetLab-2: Wet Lab RNA SmartCycler Providing PCR Capability on ISS.

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