Targeting the Kinase-Independent Pro-survival Function of EGFR in Prostate Cancer.

摘要

The proposed study, targeting the kinase independent pro-survival function of EGFR in prostate cancer, is to investigate the molecular mechanism of EGFR-SGLT1 interaction and test the possibility of use interfering peptides to disrupt the EGFR-SGLT1 interaction for therapeutic purpose, and in parallel to profile the expression status of EGFR/SGLT1 in prostate cancer tissues. Outcomes from this study may lead to find novel strategies for EGFR targeted therapy for prostate cancer. To date, we have successfully constructed expression plasmids for 12 type of truncated EGFR, which are the key tools to map the interaction domains between EGFR and SGLT1, and we have successfully developed/characterized a polycolonal antibody against human SGLT1 for immunihistochemical staining of human prosate tissues.