Protein Neo-Antigens in Breast Cancer by Combinatorial Phage Technology

摘要

Combinatorial phage technology is used to identify and characterize newlyexpressed proteins which elicit plasma cell reactions in certain breast carcinomas. It is our principal hypothesis that this reaction reflects a local immune response against tumor that is etiologic in the historical impression of a more favourable natural course of these tumors after surgery-only therapy. DNA sequencing of IgG H and L chains from random unselected clones indicates a dramatic focus in libraries derived from two patients with medullary ductal carcinoma, supporting our hypothesis of a local immune response in these tumors. Reactive phage clones are selected from phage display libraries using optimal cell-based panning conditions with medullary ductal carcinoma cells. It was demonstrated that Her2/neu and p53 are not the eliciting antigens. We will be employing immunoprecipitation and immunoblotting in order to isolate the antigen of interest.