Biosensor-Based Detection and Verification System for Bio-Chemical WarfareAgents, Appendices 1, 2 and 3

摘要

The purpose of this contract was to explore and demonstrate the application oftechnology based on the Light Addressable Potentiometric Sensor (LAPS) to detection and verification problems for bio-chemical warfare agents. The principal analytical method employed was microphysiometry, by which perturbations of cell physiology are detected using the LAPS. The work was done in three principal segments. First, silicon microtechnology was used to design and fabricate sensor chips and microfluidic components suitable for the project. Second, a systems-engineering effort designed and assembled a prototype high-performance microphysiometer. Third, a biological effort employed a wide variety of cellular and ligand-receptor model systems to validate the generality of the method and the performance of the prototype instrument.