Fabrication of High Affinity Synthetic Ligands for Microbes

摘要

To define their carbohydrate binding specificity, a variety of Vibrio cholerastrains were tested for their ability to (a) hemagglutinate red blood cells in a carbohydrate-inhibitable manner; (b) bind radiolabeled multivalent carbohydrate ligands; and (c) aggregate in response to multivalent carbohydrates. Although hemagglutination and aggregation were demonstrated, reproducible carbohydrate specificity was not apparent. Binding of E. histolytica membranes to specific carbohydrates was characterized. High affinity binding to clustered, multivalent GalNAc residues was demonstrated. Highest affinity was obtained when >%O GalNAc residues were synthetically clustered by covalently attaching them to a carrier protein (bovine serum albumin). Binding of GalNAc to the E. histolytica lectin