Modification of the In Vitro Hydra Assay Developmental Toxicity Screen forEvaluation of Airborne Toxicants: Assessment of Ammonium Perchlorate and Vapor Expos

摘要

When the Combat Exclusion Law was repealed, military women's duties were expandedto include duties that formally were exclusively performed by men. These military women are occupationally exposed to chemicals, including airborne toxicants, for which no developmental or reproductive toxicity testing has been performed. The reproductive toxicity of these chemical compounds needs serious consideration so that military women can continue to perform their mission responsibilities and remain healthy. An in vitro developmental toxicity screen was performed to determine the developmental hazard index (A/D ratio) for ammonium perchlorate (AP), tricresyl phosphate vapor phase lubricant (TCP), and diethyleneglycol monomethylether (DGME) using the hydra assay. The screen employed exposing both adult Hydra attenuate and 'artificial embryos' composed of disassociated hydra cells to these military compounds to investigate potential developmental toxicity. AID ratios of 1.71, <0.055, and 1.75, obtained for AP, TCP, and DGME, respectively, would indicate that these military compounds should not be considered primary developmental toxins in the context of this assay.