Trace Analysis of Zn(II), Be(II) and Bi(III) by Enzyme-CatalyzedChemiluminescence

摘要

A novel technique for the trace analysis of metal ions Zn(II), Be(II) and Bi(III)in bulk solutions is discussed. This technique involves the generation of chemiluminescence signal from alkaline phosphatase catalyzed hydrolysis of a phosphate derivative of 1,2-dioxetane. Zn(II) can be determined by two methods, reactivation of an apoenzyme and inhibition of the native enzyme. Be(II) and Bi(III) can also be determined quantitatively by inhibition of the native enzyme. Sub-ppb to ppm level detection of Zn(II), Be(II) and Bi(III) has been achieved. Initial studies with mixed metals are also reported. The technique described is rapid, sensitive and can be readily applied to the microassay of heavy metal ions.