Systemic Bacteraemia following Hemorrhagic Shock in Mice: Alleviation with OralInterleukin-6

摘要

A murine model of hemorrhagic shock was used to investigate bacterialtranslocation from the gut and subsequent systemic immunoreduction. Anesthetized mice were bled from the femoral artery, and held at a mean arterial blood pressure of 35 mm Hg for one hour then resuscitated with shed blood and two-fold volume lactated Ringe's solution. Upon awakening, they were given cytokines or control media orally. Bacteriological cultures of livers, spleens and mesenteric lymph nodes from hemorrhged mice given cytokine had significantly fewer bacteria/gm of tissue than those given media. Recombinant IL-6 mimicked the effects seen with crude cytokines. Reduction of proliferation among spleen cells from hemorrhaged mice was observed and could be partially returned to normal by cytokine feeding. Mixing experiments in which cells from hemorrhaged mice were added to those of normal mice in an MLR showed no suppressor activity.