Noninvasive Ambulatory Assessment of Cardiac Function and Myocardial Ischemia inHealthy Subjects Exposed to Carbon Monoxide. Special Study: Trichloroethylene Exposure in Women and Men

摘要

The purpose of this study was to conduct controlled human trichloroethylene (TCE)inhalation studies to support physiologically-based pharmacokinetic (PB-PK) modeling of TCE exposure in healthy men and women. TCE is a volatile liquid used in degreasing operations, industrial cleaning, paint strippers, rug cleaners, spot removers, and typewriter correction fluid with widespread industrial and military use. Eight female and nine male subjects willing to provide informed consent were medically qualified for participation, then were exposed to 4 hours of 50 ppm or 100 ppm TCE at rest in a controlled environmental chamber. Following exposure, subjects remained in the chamber breathing clean air at rest and during sleep for an additional 18 hours. Periodically during and after exposure, blood samples were taken via venous catheter and urine samples were collected via specimen containers. The blood and urine samples were partitioned and treated with reagents, and the subsamples flash-frozen. Frozen samples were shipped to designated laboratories for chemical analysis, and the resulting TCE and TCE-metabolite data transmitted to the Tri-Service Toxicology group at Wright-Patterson Air Force Base (WPAFB) for PB-PK model analysis. Results of the TCE PB-PK model analyses are to be reported elsewhere by the WPAFB investigators.