Effects of White Cell Filtration, ACD Concentration and Rotation During Collection, Storage and Cryopreservation of Platelet Concentrates

摘要

This study was performed to assess methods for the enumeration of intact platelets, platelet aggregates and platelet microparticles in fresh platelets, platelets stored at 22 deg C, and cryopreserved platelets. The methods used included phase microscopy, the Coulter electronic impedance method, and flow cytometry using log forward light scatter. Also measured was the platelet aggregation response to agonists in vitro, platelet production of thromboxane B2 following stimulation with agonists in vitro, platelet surface markers prior to and following in vitro response to different agonists, platelet response to hypotonic stress, and plasma pH, pCO2, pO2 thromboxane B2, and complement C3a. Single donor platelets were collected from healthy volunteers by a plateletpheresis procedure using the Haemonetics V50 with surge. The initial phase of the study was performed to assess the optimum time to use the Pall leukocyte reduction filter Number 10 to produce leukoreduced platelets. In this study, single donor platelets were collected using the Haemonetics V50 at an ACD to blood ratio of 1:8 and the platelets were filtered immediately after collection, after storage at 22 deg C for 4 hours with rotation and after storage at 22 deg C for 5 days with rotation.