Role of Scavenger Receptors in Uptake of uPA:PAI-1 Complexes in Breast Cancer

摘要

This proposal focuses on a novel topic that had not been investigated previously--i.e., potential derangements in expression or trafficking of scavenger receptors, LRP and megalin, and their interactions with uPA-R, uPA and PAI-1 in breast cancer cells. Upon beginning this work, our first order of business was to establish the systems required to carry out the proposed studies and to use them to evaluate the levels of expression of scavenger receptors in breast cancer vs normal cell lines. During the first year of the grant we have prepared several essential reagents (antibodies) and have characterized four cell lines -two estrogen-insensitive and one estrogen-sensitive breast cell lines and one normal mammary cell line.