Acoustic Microsensors II. Detection of Staphylococcal Enterotoxin B Employing aPiezoelectric Crystal Immunosensor

摘要

This paper reports on a study concerning the development of a biochemical sensorfor Staphylococcal Enterotoxin B (SEB), a substance which falls within the bracket of biological warfare agents. A 20 MHz piezoelectric quartz crystal sensor device was employed in a flow injection system. The assay for SEB is based on a competition scheme using polyclonal antibodies (anti-SEB). Three parameters, i.e. the flow rate in the flow cell, the incubation time and the anti-SEB concentration, were optimised. A detection limit of 0.1 micrograms/ml is obtained, whereas at concentrations of SEB of 10 micrograms/mi or higher the sensor response is completely inhibited. The results were compared with a competition Enzyme Linked Immunosorbent Assay (ELISA).