New Approaches to Evaluate the Biological Degradation of RDX in Groundwater.

摘要

The primary goal or this SERDP project was to combine state-of-the art analytical techniques, molecular approaches, and biogeochemical studies to enhance ow understanding of the biodegradation of hexahydro-1 ,3,5-trinitro-1 ,3,5-triazine (RDX) in groundwater. New methods were developed and tested to preserve key RDX metabolites, and the presence of these metabolites was examined at several DoD sites. Microcosm and mesocosm studies with aquifer samples were conducted to assess pathways and rates of RDX degradation under differing geochemical conditions. The microbial communities involved in degradation under these conditions were then characterized using stable isotope probing (SIP) with both 13C-labeled and 15N-labeled RDX. A wide array of organisms, many of which have not previously been associated with l