Genetic Determinants of Sindbis Virus Oral Infectivity in Aedes AegyptiMosquitoes

摘要

The TE/3'2J double subgenomic Sindbis viruses (dsSIN) have been used to expressgenes in Aedes Aegypti nerve and salivary gland tissues. However, TE/3'2J viruses must be intrathoracically inoculated into the mosquitoes and exogenous expression in the mosquito midgut has not been possible. To improve oral infectivity, serial oral passage with selection for variants that disseminated to neural tissues resulted in an eight-fold increase in dissemination rate and enhanced tropism for