Cloning Human Chromosome 17 Genes: Candidate Genes for BRCA1

摘要

Our research interest is focused on the identification of genes from chromosome 17. The isolation of genes transcribed from chromosome 17 will provide candidates for the proposed sporadic breast cancer genes and genes for other human disorders.The ability to isolate genes in a chromosome specific manner provides simultaneous identification of the expressed sequence and a chromosomal location. Our approach identifies expressed sequences by reciprocal probing of arrayed cDNA libraries and a chromosome specific cosmid library. To date from the cDNAs isolated from human chromosome 17 we have identified two very important gene One gene, which encode a coactosin like protein (CLP) and maps to 17p11.2 has been demonstrated by us and our collaborators to be involved in the Smith-Magenis Syndrome, a neuro-muscular disorder. A second gene which encodes a putative transcription factor has been demonstrated by my laboratory to be a key gene in the mammalian circadian rhythm pathway. Based on protein homology, a second circadian gene was recently identified by my laboratory.