Cell Regulation By a Novel Estrogen Regulated Gene

摘要

This report describes the work conducted in an expiring 2y IDEA for which a no cost extension was approved. Our original proposal was funded to study a human estrogen regulated transcript, HEM45. Recent homology studies indicate that HEM45 is related to a family of proteins that have an exonuclease domain. These include DNA proof-reading proteins and proteins associated with mitotic structures. Studies in the project have demonstrated altering patterns of HEM45 distribution detectable in the cell cycle and an association with the mitotic spindle. These data support a role for HEM45 in the cell cycle/ proliferation, however we have not been able to significantly perturb levels of native HEM45 transfected fusion protein in cells and cell extracts.