Preventive Role of Specific Dietary Factors and Natural Compounds Against DNA Damage and Oxidative Stress

摘要

I have developed a series of reagents and a luciferase-based functional assay for identifying and testing ligands for Notch4 receptor. I am in the process of testing several candidate genes for their ability to activate Notch4 receptor. In addition, I identified a novel negative regulator of Notch activity, SEL-10. Through biochemical studies, I have shown that SEL-10 interacts with Notch in a domain specific and probably phosphorylation-dependent manner. I also provided some evidence indicating that SEL-10 can regulate Notch ubiquitination in the cell. These results support my model that SEL-10 inhibits Notch activity by targeting it for ubiquitin-mediated protein degradation.