Production of Normal Human Epidermal Keratinocytes for Use in Biochemical Research

摘要

This technical report outlines procedures that have been developed to provide a cost effective and high efficiency quality control quantity of normal human epidermal keratinocyte (NHEK) cells to conduct research into the mechanism of action of chemical warfare (CW) agents and to reduce the dependence on live animal testing with toxic CW agents. Adult NHEK cell lines were grown at different cell densities in a variety of diverse tissue culture vessels. The purpose of this technical report is to provide an overall review of important tissue culture parameters for the optimal production and use of NHEK in toxicological research. These cells have provided valuable information for the design of therapeutic intervention against 2,2'-dichlorodiethyl sulfide (sulfur mustard, HD) induced lesions.